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KMID : 0360220170580030327
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Journal of the Korean Ophthalmological Society
2017 Volume.58 No. 3 p.327 ~ p.332
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Effect of Trypan Blue on the Survival of Cultured Trabecular Meshwork Cells
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Kang Hye-Ri
Hwang Seok-Jin
Kim Jae-Woo
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Abstract
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Purpose: To evaluate the effects of trypan blue (TB) on the survival of cultured human trabecular meshwork cells (HTMCs).
Methods: Primarily cultured HTMCs were exposed to 0.05, 0.10 or 0.50% TB for 1, 5 or 30 min. Cellular survival was assessed using the MTT assay and degree of apoptosis was analyzed with flow cytometry using annexin-V/propidium iodide double staining.
Results: Long-term exposure or high concentration of TB decreased the survival of HTMCs (p < 0.05). In flow cytometric analysis, exposure to 0.50% TB for 30 min increased the degree of apoptosis (p < 0.05). Commercial TB decreased cell survival after exposure for 5 min and increased the degree of apoptosis after exposure for 30 min (p < 0.05).
Conclusions: TB may cause cellular damage of cultured HTMCs and apoptosis could be the underlying mechanism. In
TB-assisted cataract surgery, TB should be used for the shortest time possible and removed completely.
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KEYWORD
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Apoptosis, Flow cytometry, Survival, Trabecular meshwork cell, Trypan blue
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